Not an expert here, but having been through this very recently, your business is already an asset which will need to be considered in the financial settlement. In preparation for the financial arrangements you will both need to complete a form E which lists ALL or your assets. This includes businesses, jewellery, cars etc etc as well as property and savings.
You would be well advised to discuss this decision with either your solicitor, if you have one, or with the mediation service advisor if you are going down that route.
I see you are in Australia. Unfortunately most of us here don''t know much about Australian law so any advice you get is likely to be unreliable. You need to talk to a local lawyer, unless you are divorcing here.